The Cremer Lab

Head: Prof. Dr. Christoph Cremer
Deputy Group Leader: Dr. Udo Birk

IPMB Heidelberg

IMB Mainz

Cooperation Unit Biophysics of the Genome Structure

Superresolution Microscopy

Welcome to eXtreme resolution microscopy

Our group at the Institute of Molecular Biology GmbH - an Excellence Centre for Life Sciences funded by the Boehringer Ingelheim Foundation - and at the Kirchhoff Institute for Physics focusses on development and application of novel far field fluorescence microscopy techniques. We set up LASER microscopes i.e., work on optical setups and on image analysis tools for quantitative (fluorescence) microscopy with a detection sensitivity down to the single molecule level.

Conventional epifluorescence microscopy is limited in resolution (to about 200 nm laterally, 600 nm axially) by the shere nature of light (by diffraction), and is hence insufficient to study the nanostructure of subcellular components. At IMB-Mainz, we have established a variety of superresolution microscopy ("nanoscopy") methods. Our present spectrum for 'nanoimaging' of nuclear structures comprises confocal laser scanning 4Pi-microscopy, Spatially Modulated Illumination (SMI) and Patterned Excitation Microscopy (PEM) devices, as well as Spectrally Assigned Localization Microscopy (SALM). Our microscope systems can and have been applied to study the composition, function and metabolism of many biomolecular structures. Few other fields of biology benefit as much from the revolutionary developments in light microscopy as the study of the architecture of the cell nucleus and of its functional layout.

In the multi-disciplinary environment here at IMB-Mainz we also offer Masters and PhD theses at the utmost forefront of what is currently possible in far field light microscopical analyses of the cell. Depending on the background and interest of the candidate, the focus of the PhD will be shifted either towards biophysics/optics or towards biology.

A brief overview of our work - in particular of the (mainly biological) applications of these eXtreme resolution microscopes - can also be found on the IMB-website of our group.

Our latest work


Dr. Aleksander Szczurek
obtains his PhD with summa cum laude. CONGRATULATIONS
Prof. Rainer Heintzmann
Jena University
10:00 IMB SR 2nd floor
Lightwedge and Lightsheet-Raman Microscopy
Dr. Michael Blank
Bar-Ilan University
14:00 IMB SR 2nd floor
The role of E3 ubiquitin ligase Smurf2 in cancer
11:00 IMB Room 00.322
Workshop "Super-resolution microscopy (SRM) of human DNA regions"
more …


Prof. C. Cremer
Dr. U. Birk
Institute of Molecular Biology
Ackermannweg 4
D-55128 Mainz, Germany
Fax: ++49-6131-39-21421
Tel.: ++49-6131-39-21524