M.Sc./M.Ed. Seminar: Fundamental Physical Problems in Optical Imaging


Summer term 2014/2015, Begin: Apr. 20.

Times: Wednesday 11:00-12:30,
Place: IMB Mainz, Ackermannweg 4, Meeting room 2.624 (2nd floor)

!!! Updated schedule: meeting room 02.624 !!!
Times: Wednesday 11:00-12:30.

Seminar available through video conferencing at KIP, Uni Heidelberg
Lecturers: Dr. Udo Birk, Prof. Dr. Christoph Cremer
The language used throughout the course will be English, unless otherwise specified.
This course will be taught at the post-baccalaureate level. A basic knowledge in electromagnetics/optics and in digital image processing could be very helpful. These are subject of our course on Basics of Biomedical Optics.

In this seminar, some of the apparently obvious but on closer look not so trivial questions related to optical imaging, i.e. the use of light as a contrast mechanism as well as for detection on a light detector will be discussed. Discussions will be stimulated and augmented by regular presentations of recent research papers.

Students actively participating will give a presentation focusing on one of the particular problems encountered in "real" optical imaging, i.e. in the presence of factors deteriorating the image quality from the ideal "theoretical" image. For example, the influence of noise on quantitative imaging, of motion, of fluctuating light output etc. will be discussed. Since the aim of optical imaging is (at least in a physics environment) to obtain quantitative data, problems related to image quantization and image digitization (pixelated digital images), as well as to image processing are a further important focus of this seminar.

These general topics will be amended by more specialized presentations on problems related to fluorescence imaging such as e.g. photophysics of fluorophores, and to superresolution microscopy.

Aim of this seminar is to provide the students with the opportunity to prepare for oral examinations by providing a discussion forum for state-of-the art optical imaging methods. At the same time, topics of the seminar presentations will illustrate the enormous potential that photonics and in particular optical imaging has for your future career.

M.Sc. Physics course

Jogustine entry for this course at the University of Mainz

Course material

Will be handed out during the lectures/course

Preliminary program

Date 1. Technical Presentation by 2. Journal Article by
20.04. General outline of the course
Registration for seminar talks
UB --- ---
27.04. Imaging polymers NN NN
04.05. Superresolution in biology NN NN
11.05. Sampling and digitization NN NN
18.05. Effects of Illumination in Fluorescence Microscopy: Forces, Damage, and Polarization NN NN
25.05. Stimulated Emission in real experiments NN NN
01.06. How to construct a microscope objective lens NN NN
08.06. Bessel beams NN NN
15.06. Discussion

22.06. Multiblinking in Localization Microscopy NN NN
29.06. Numerical Aperture: How it defines image quality NN NN
06.07. Multi-photon absorption and fluorescence NN NN
13.07. Structured Illumination Microscopy NN NN
20.07. Discussion

Additional topics for the talks could be:
- Spatially Modulated Illumination
- Optical Tomography