M.Sc./M.Ed. Seminar: Fundamental Physical Problems in Optical Imaging


Winter term 2014/2015, Begin: Oct. 27.

Times: Monday 14:15-15:45,
Place: IMB Mainz, Ackermannweg 4, Meeting room 1.624 (1st floor)
Seminar available through video conferencing at KIP, Uni Heidelberg
Lecturers: Dr. Udo Birk, Prof. Dr. Christoph Cremer
The language used throughout the course will be English, unless otherwise specified.
This course will be taught at the post-baccalaureate level. A basic knowledge in electromagnetics/optics and in digital image processing could be very helpful. These are subject of our course on Basics of Biomedical Optics.

In this seminar, some of the apparently obvious but on closer look not so trivial questions related to optical imaging, i.e. the use of light as a contrast mechanism as well as for detection on a light detector will be discussed. Discussions will be stimulated and augmented by regular presentations of recent research papers.

Students actively participating will give a presentation focusing on one of the particular problems encountered in "real" optical imaging, i.e. in the presence of factors deteriorating the image quality from the ideal "theoretical" image. For example, the influence of noise on quantitative imaging, of motion, of fluctuating light output etc. will be discussed. Since the aim of optical imaging is (at least in a physics environment) to obtain quantitative data, problems related to image quantization and image digitization (pixelated digital images), as well as to image processing are a further important focus of this seminar.

These general topics will be amended by more specialized presentations on problems related to fluorescence imaging such as e.g. photophysics of fluorophores, and to superresolution microscopy.

Aim of this seminar is to provide the students with the opportunity to prepare for oral examinations by providing a discussion forum for state-of-the art optical imaging methods. At the same time, topics of the seminar presentations will illustrate the enormous potential that photonics and in particular optical imaging has for your future career.

M.Sc. Physics course

Jogustine entry for this course at the University of Mainz

Course material

Will be available here shortly ...

Preliminary program

Date 1. Technical Presentation by 2. Journal Article by
27.10. General outline of the course
Registration for seminar talks
UB --- ---
03.11. Software FastSPDM UB -  
10.11. Nadine Waltrich: Her2 receptors measured by SMLM NW Fluorescence Complementation in SMLM
17.11. Fluorescence: Excitation and emission statistics FS Adaptive Optics CZ
24.11. Photophysics of fluorophores AS RESOLFT nanoscopy with more than 100,000 'doughnuts'
01.12. Laser light and laser calibration and specification DP HL
08.12. Discussion

15.12. Discussion

22.12. Discussion

29.12. Winter break

05.01. Winter break

12.01. CARS GB - DW
19.01. Detectors: from photons to counts & how to calibrate a detector JN - KP
26.01. Superresolution in biology HL - FS
02.02. How to determine resolution and how to experimentally measure PSF KP - DP
09.02. Sampling and digitization DW - JN

Additional topics for the talks could be:
Multiphoton processes
Localization Microscopy
Structured Illumination Microscopy