Basics of Biomedical Optics / Grundlagen der Biomedizinischen Optik

Lecture / Tutorial / Lab

Winter term 2014/2015, Begin: Oct. 14.

Lectures: Tuesday 14:15-15:45, IMB Mainz, Ackermannweg 4, Seminar room 2nd floor
Lectures (only) available through video conferencing at KIP, Uni Heidelberg
Tutorial: Wednesday 13:15-15:00, Institut für Physik, Staudingerweg 7, CIP pool 03.423
Lab: Block course March 09-11, three full days.
Tutorial and Practical Lab Course is for Students in Mainz only.
Lecturers: Dr. Udo Birk, Prof. Dr. Christoph Cremer, Prof. Dr. Michael Hausmann
Teaching language will be English, unless otherwise specified.

In the tutorials, a general introduction to the concept of image processing will be given, based on the programming environment MATLAB. Students will be given exercises to model the physical processes discussed during the lectures, and to evaluate the potential of the corresponding imaging / illumination / detection techniques.

In the lectures to Basics of Biomedical Optics 1 (winter term 2014/2015), i.e. in the first half of this two semester course, the main optical concepts of imaging and general technical aspects for realization of optical imaging devices are in focus. After an introduction to the electromagnetic concepts of light and its interaction with tissue, we will discuss recent development in technical optics (e.g. light sources, detectors) making possible the huge progress that biomedical imaging has recently seen. Several conceptual models representing (and simplifying) the optical image formation process will be discussed. Reasons for limitations in image resolution will be given, and means to overcome the technical problems will be presented. Contrasting mechanisms of optical imaging will be discussed and first approaches to enhance the 3D resolution in microscopy. The course will be complemented by a brief historical outline.

In the labcourse, several examples of high- and super-resolution microscopes will be worked with in hands-on experiments. These microscopes will be applied to image biological specimens from ongoing research collaborations. Students will perform image analysis to reconstruct represenations of the biological structures at hand.
The aim is to provide the students with access to state-of-the art far field optical microscopy setups, illustrating the enormous progress that far field optical microscopy has recently made.

M.Sc. Physics course

Jogustine entry for this course at the University of Mainz

Course lecture material and exercises

available here ...

Preliminary program

Date Content Lecturer
07.10. no lectures UB
14.10. Optional opening lecture
A short history of optics (glass, lenses, microscopes, telescopes, ...)
21.10. What is color, what is scattering?
The electromagnetic spectrum and the statistical nature of light
28.10. Classical optics, from ray optics to wave optics to beam optics, some quantum optics
Gaussian beam propagation
04.11. Light Sheet Microscopy/FCS Malte Wachsmuth
11.11. Fourier Optics UB
18.11. EM Dipole: near field, far field
near field imaging
25.11. Lasers: Theory, conditions, and technical realization UB
02.12. Detectors
Optical Design
09.12. History 1: The Advent of Microscopy CC
16.12. Confocal Laser Scanning Microscopy SR+MH
23.12. Winter break
30.12. Winter break
06.01. MZ: lab tour / HD: Winter break UB
13.01. Optical Imaging 1: Image formation and Point Spread Function UB
20.01. Optical Imaging 2: Limitations in imaging
Apodization, Vectorial Diffraction Theory
27.01. White light generation
Fluorescence and other contrasting mechanisms
03.02. Axial Imaging: Micro-Axial Tomography, Theta Microscopy UB
10.02. HD: term ended.
Interaction of light and tissue: effects of laser power, pulse parameters